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Image Search Results
Journal: PLoS ONE
Article Title: The Breadth, but Not the Magnitude, of Circulating Memory B Cell Responses to P. falciparum Increases with Age/Exposure in an Area of Low Transmission
doi: 10.1371/journal.pone.0025582
Figure Lengend Snippet: AMB were defined as being CD19 + CD21 − CD27 − CD10 − , classical MBC were defined as CD19 + CD21 + CD27 + . The gating strategy is shown in (A). Viable PBMC were gated using FSC/SSC (not shown) and then displayed according to CD19 + expression. The CD19 + population was displayed according to their expression of CD21 and CD27. To identify atypical MBCs, CD10 − cells were subsequently selected from CD21 − CD27 − cells, using a CD10 gate defined on the entire PBMC population. (B) Percentages of CD19 + cells expressing the atypical and classical MBC phenotype are shown for children <5 and >5 years of age. P-values are given for the Mann-Whitney test.
Article Snippet: The cells were stained with the following fluorochrome-labelled mouse anti-human antibodies: ECD-anti-CD19 (Beckman Coulter), and APC-anti-CD21,
Techniques: Expressing, MANN-WHITNEY
Journal: PLoS ONE
Article Title: Natural Killer Cells Mediate Protection against Yersinia pseudotuberculosis in the Mesenteric Lymph Nodes
doi: 10.1371/journal.pone.0136290
Figure Lengend Snippet: (A) Mice were infected with 2 x 10 7 CFU of Y . pseudotuberculosis strain YPIII. Three days post infection mLN were excised and NK cell population distribution was analyzed by flow cytometry. To do so, cells were stained with Live/Dead, CD3, NK1.1, CD11b, CD27, CD69 and CD107a. Grey filled squares represent uninfected mice, black filled circles illustrate Y . pseudotuberculosis infected mice. CD11b + , CD27 + NK cells from the mLN were further analyzed for their expression profiles of the surface markers CD107a (B) and CD69 (C). Grey filled squares represent uninfected mice, while black filled circles represent YPIII infected mice. Data from three independent experiments were pooled and analyzed with a Student’s t-test (*, p < 0.05; ***, p<0.001).
Article Snippet: Subsequently, 3 x 10 6 cells were surface-stained with the following antibodies: Live/Dead Fixable Blue Dead Cell Stain (Invitrogen), CD3:v450 (BD Biosciences, cl.17A2), Ly6G:PE-Cy7 (eBioscience,cl.1A8), CD16/CD32 (BD Biosciences, cl.93), CD4:PerCp-Cy5.5 (eBioscience, cl.RM4-5), CD8a:PE (eBioscience, cl.53-6.7), CD11b:ef450 (eBioscience, cl.M1/70), CD11b:Brilliant Violet 605 (Biolegend, cl.M1/70), CD11c:APC-ef780 (eBioscience, cl.N418), F4/80:PE (eBioscience, cl.BM8), CD19:FITC (eBioscience, cl.N418),
Techniques: Infection, Flow Cytometry, Staining, Expressing